BMRB Entry 5808

Click here to enlarge.

PDB ID: 1pd7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5808
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Chemical shift assignments and relaxation parameters for the PAH2 domain of mSin3B bound to an extended SID of Mad1

Deposition date:

2003-05-22

Original release date:

2004-02-12

Authors:

van Ingen, Hugo; Jansen, Jaap; Spronk, Chris; Vuister, Geerten

Citation:

Citation: van Ingen, Hugo; Lasonder, Edwin; Jansen, Jaap; Kaan, Anita; Spronk, Chris; Stunnenberg, Henk; Vuister, Geerten. "Extension of the binding motif of the Sin3 Interacting Domain of the mad family proteins" Biochemistry 43, 46-54 (2004).
PubMed: 14705930

Assembly members:

Assembly members:
PAH2 domain, polymer, 105 residues, Formula weight is not available
Mad1, polymer, 24 residues, 2973 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PAH2 domain: ESDSVEFNNAISYVNKIKTR FLDHPEIYRSFLEILHTYQK EQLHTKGRPFRGMSEEEVFT EVANLFRGQEDLLSEFGQFL PEAKRSLFTGNGSAEMNSGQ KNEEK
Mad1: VRMNIQMLLEAADYLERRER EAEH

Data sets:

assigned_chemical_shifts
- shift_set_1
order_parameters
- S2_parameters_label
heteronucl_T1_relaxation
- 15N_T1_Set_1
heteronucl_NOEs
- heteronuclear_NOE_1

Data type	Count
1H chemical shifts	552
13C chemical shifts	269
15N chemical shifts	90
T1 relaxation values	78
order parameters	77

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PAH2 domain of mSin3B	1
2	SID domain of Mad1	2

Entities:

Entity 1, PAH2 domain of mSin3B 105 residues - Formula weight is not available

1

GLU

SER

ASP

SER

VAL

GLU

PHE

ASN

ALA

2

ILE

SER

TYR

VAL

ASN

LYS

ILE

LYS

THR

ARG

3

PHE

LEU

ASP

HIS

PRO

GLU

ILE

TYR

ARG

SER

4

PHE

LEU

GLU

ILE

LEU

HIS

THR

TYR

GLN

LYS

5

GLU

GLN

LEU

HIS

THR

LYS

GLY

ARG

PRO

PHE

6

ARG

GLY

MET

SER

GLU

VAL

PHE

THR

7

GLU

VAL

ALA

ASN

LEU

PHE

ARG

GLY

GLN

GLU

8

ASP

LEU

SER

GLU

PHE

GLY

GLN

PHE

LEU

9

PRO

GLU

ALA

LYS

ARG

SER

LEU

PHE

THR

GLY

10

ASN

GLY

SER

ALA

GLU

MET

ASN

SER

GLY

GLN

11

LYS

ASN

GLU

LYS

Entity 2, SID domain of Mad1 24 residues - 2973 Da.

1

VAL

ARG

MET

ASN

ILE

GLN

MET

LEU

GLU

2

ALA

ASP

TYR

LEU

GLU

ARG

GLU

ARG

3

GLU

ALA

GLU

HIS

Samples:

sample_1: Mad1 1.3 mM; PAH2 domain, [U-13C; U-15N], 1.3 mM; K2HPO4/KH2PO4 buffer 50 mM

Ex-cond_1: pH: 6.3; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	sample_1	not available	Ex-cond_1
HNCACB	sample_1	not available	Ex-cond_1
CBCACONH	sample_1	not available	Ex-cond_1
(H)CCH-TOCSY	sample_1	not available	Ex-cond_1
15N NOESY	sample_1	not available	Ex-cond_1
13C NOESY	sample_1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Varian UnityInova 500 MHz
Varian UnityInova 600 MHz
Varian UnityInova 750 MHz
Varian UnityInova 800 MHz

PDB	1G1E 1PD7 1S5Q 1PD7
DBJ	BAG35605 BAG73371 BAH13406 BAG35605
EMBL	CAG38734 CAG46493 CAG46493 CAG38734
GB	AAA36194 AAH69377 AAH69433 AAH98396 AAI13532
REF	NP_001179309 NP_001189442 NP_001189443 NP_001247787 NP_002348 XP_592508 XP_525776 XP_001493078 XP_001098088 NP_002348
SP	Q05195
TPG	DAA24557
SWISS-PROT	Q05195
GenBank	AAI13532 AAH98396 AAH69433 AAH69377 AAA36194
BMRB	5457 4635
AlphaFold	Q05195