BMRB Entry 5457
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5457
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: van Ingen, Hugo; Tessari, Marco; Vuister, Geerten. "A 3D doubly sensitivity enhanced X-filtered TOCSY-TOCSY experiment" J. Biomol. NMR 24, 155-160 (2002).
Assembly members:
Mad1, polymer, 24 residues, 2973 Da.
mSin3B, polymer, 105 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Mad1: VRMNIQMLLEAADYLERRER
EAEH
mSin3B: ESDSVEFNNAISYVNKIKTR
FLDHPEIYRSFLEILHTYQK
EQLHTKGRPFRGMSEEEVFT
EVANLFRGQEDLLSEFGQFL
PEAKRSLFTGNGSAEMNSGQ
KNEEK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 169 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PAH2 domain of mSin3B | 2 |
| 2 | SID domain of Mad1 | 1 |
Entities:
Entity 2, PAH2 domain of mSin3B 105 residues - Formula weight is not available
| 1 | GLU | SER | ASP | SER | VAL | GLU | PHE | ASN | ASN | ALA | ||||
| 2 | ILE | SER | TYR | VAL | ASN | LYS | ILE | LYS | THR | ARG | ||||
| 3 | PHE | LEU | ASP | HIS | PRO | GLU | ILE | TYR | ARG | SER | ||||
| 4 | PHE | LEU | GLU | ILE | LEU | HIS | THR | TYR | GLN | LYS | ||||
| 5 | GLU | GLN | LEU | HIS | THR | LYS | GLY | ARG | PRO | PHE | ||||
| 6 | ARG | GLY | MET | SER | GLU | GLU | GLU | VAL | PHE | THR | ||||
| 7 | GLU | VAL | ALA | ASN | LEU | PHE | ARG | GLY | GLN | GLU | ||||
| 8 | ASP | LEU | LEU | SER | GLU | PHE | GLY | GLN | PHE | LEU | ||||
| 9 | PRO | GLU | ALA | LYS | ARG | SER | LEU | PHE | THR | GLY | ||||
| 10 | ASN | GLY | SER | ALA | GLU | MET | ASN | SER | GLY | GLN | ||||
| 11 | LYS | ASN | GLU | GLU | LYS |
Entity 1, SID domain of Mad1 24 residues - 2973 Da.
| 1 | VAL | ARG | MET | ASN | ILE | GLN | MET | LEU | LEU | GLU | ||||
| 2 | ALA | ALA | ASP | TYR | LEU | GLU | ARG | ARG | GLU | ARG | ||||
| 3 | GLU | ALA | GLU | HIS |
Samples:
sample_1: Mad1, [U-15N; U-13C], 1.3 mM; mSin3B 1.3 mM; potassium phosphate 50 mM
Ex-cond_1: pH: 6.3; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| The use of a 3D TOCSY-TOCSY was required to solve high degree of overlap in | sample_1 | not available | Ex-cond_1 |
| the 2D TOCSY spectrum. The overlap is caused by the highly redundant amino | sample_1 | not available | Ex-cond_1 |
| acid composition of the C-terminal part of the SID | sample_1 | not available | Ex-cond_1 |
Software:
NMRPipe -
Xeasy -
NMR spectrometers:
- Varian UnityInova 600 MHz
- Varian UnityPlus 800 MHz