The file contains assignments for ORF57 8-120 bound to REF 54-155 at 303k

Structural basis for the recognition of cellular mRNA export factor REF by herpes viral proteins HSV-1 ICP27 and HVS ORF57.

REF fragment containing stable RRM domain
Residues 54-155 are correctly numbered for wild type sequence

Construct contains the following non-REF sequences:
N-terminal T7 tag: MASMTGGQQMGRDP (assigned residue numbers 40-53)
C-terminal His tag: LEHHHHHH (assigned residue numbers 156-163)

Residues 8-120 are correctly numbered for wild type sequence
The construct contains the following non-ORF57 sequence at N-termini: MASMTGGQQMGRDP (assigned residue numbers -4 to 7) Plus the following at C-termini: LEHHHHHH (assigned residue numbers 121 to 128)

Rrm Domain Of Mrna Export Adaptor Ref2-I Bound To Hsv-1 Icp27 Peptide

Rrm Domain Of Mrna Export Adaptor Ref2-i Bound To Hvs Orf57 Peptide

unnamed protein product [Saimiriine herpesvirus 2]

hypothetical protein [Saimiriine herpesvirus 2]

transactivator, partial [Saimiriine herpesvirus 2]

immediate-early phosphoprotein [Saimiriine herpesvirus 1]

52K immediate-early protein - saimiriine herpesvirus 1 (strain 11)

RecName: Full=mRNA export factor ICP27 homolog; AltName: Full=52 kDa immediate-early phosphoprotein; AltName: Full=EB2 protein

HVS ORF57 8-120 backbone assignment, in complex with REF2-I 54-155.

20 mM Na phosphate buffer, pH 6.2, 50 mM NaCl, 50 mM L-Arg, 50 mM L-Glu, 50 mM 2-mercaptoethanol, 10 mM DTT, 10 mM EDTA