1H,13C,15N backbone resonance assignment for 1-164 construct of XRCC4

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

residues 1-213, cysteines mutated to alanine

1H, 13C, 15N backbone resonance assignment for the 1-164 construct of human XRCC4.

Cys93 --> Ala; Cys128-->Ala; Cys130-->Ala. Stop codon @ 165

Nterminal cleavable HisTag, ligated between NcoI and EcoRI sites

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500 uM XRCC4 Dimer; 20 mM Hepes pH 6.8, 140 mM NaCl, 1 mM EDTA, 2 mM DTT, 100 mM arginine, 100 mM glutamic acid, 0.02% NaN3