TROSY offset: amide protons and nitrogens.

Mechanism of activating mutations and allosteric drug inhibition of the phosphatase SHP2.

Residue 1-3 represent a non-native sequence to facilitate TEV cleavage.

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426).

The backbond assignment for SHP2(1-529).
Backbone 1H, 13C, and 15N Chemical Shift Assignments for SHP2 (1-529)

TROSY-versions of the listed experiments were used.

The CCPN NMR assignment and data analysis application

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Department of Biochemistry, Cambridge CB2 1GA, UK