A streamlined method for preparing split intein for NMR study.

N fragment with residue 1-102 and a C-terminal his tag C fragment with residue 103-137

Conserved Residues That Modulate Protein Trans-splicing Of Npu Dnae Split Intein

DNA polymerase III, alpha subunit [Nostoc punctiforme PCC 73102]

H6-A-IntN [Expression vector pEBDuet28A]

UmuD-T7 RNA polymerase(1-514)-NpuN [synthetic vector pCOLA-AraC-pBAD-SspC-T7_RNAP(515-884)-UmuD-T7RNAP(1-514)-NpuN]

UmuD-T7 RNA polymerase(1-514)-NpuN [synthetic vector pCOLA-AraC-pBAD-UmuD-T7_RNAP(1-514)-NpuN]

DNA polymerase III subunit alpha [Nostoc punctiforme]

Backbone and side chain 1H, 13C, and 15N chemical Shift assignments for native split Npu DnaE intein

Institute of Bioinformatics and Structural Biology, National Tsing Hua Univ., Hsinchu city, Taiwan

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax