Periplasmic binding protein. Extracellular receptor for ribose.

Same protein cristallized in the absence of ligands. Therefore it exhibits an open conformation.

Same protein cristallized in the presence of sugar ligand. Therefore it exhibits a closed conformation.

Systematic_chem_shift_offset:
13C Bruker Referencing: 'all 13C': +2.57.

Journal of the American Chemical Society
Carbohydrate affinity for the glucose-galactose binding protein is regulated by allosteric domain motions.

Periplasmic Binding Proteins bind sugars and act in the signalling cascade of the chemotactic response

RBP E.coli bound to glucose
NMR assignment of backbone: N, H, alpha C and betha C

RBP E. coli
Assignment of backbone NH, alpha and beta carbons

Ecoli RBP sugar free backbone assignment

Ecoli GGBP sugar free backbone assignment

X-ray structure of open unliganded E.coli Ribose Binding Protein

X-ray structure of closed ligand bound E.coli Ribose Binding Protein

Ecoli GGBP sugar free solution structure

1mM NCD RBP
20mM tris pH = 7
150mM NaCl 10uM CaCl2
1mM NaN3

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax