Backbone and Ile(d1), Leu and Val Sidechain Resonance Assignments of the NUDIX Domain of Yeast Dcp2

Residues 100-245 of yeast Dcp2 as C-terminal fusion to the B1 domain of protein G

Backbone and Ile(d1), Leu and Val Chemical shift assignments

mRNA decapping is promoted by an RNA-binding channel in Dcp2.

Assigned as a fusion to the B1 domain of Streptococcal protein G

Solution Structure Of Catalytic Domain Of Ydcp2

Crystal Structure Of Saccharomyces Cerevisiae Dcp2 Nudix Domain In Complex With Mg

Crystal Structure Of Saccharomyces Cerevisiae Dcp2 Nudix Domain In Complex With Mg (e153q Mutation)

Crystal Structure Of Saccharomyces Cerevisiae Dcp2 Nudix Domain (e198q Mutation)

K7_Dcp2p [Saccharomyces cerevisiae Kyokai no. 7]

suppresses the respiratory deficiency of a yeast pet mutant [Saccharomyces cerevisiae]

RecName: Full=mRNA-decapping enzyme subunit 2; AltName: Full=Protein PSU1 [Saccharomyces cerevisiae YJM789]

RecName: Full=mRNA-decapping enzyme subunit 2; AltName: Full=Protein PSU1 [Saccharomyces cerevisiae S288c]

TPA: decapping enzyme complex catalytic subunit DCP1 [Saccharomyces cerevisiae S288c]

20mM Na Phosphate
100mM Sodium Sulfate
50mM Arginine
50mM Glutamate
100mM NaCl
Sample one was made with 1H/13C glucose, 99% D2O and 15N4Cl in standard M9.

20mM Na Phosphate
100mM Sodium Sulfate
50mM Arginine
50mM Glutamate
100mM NaCl
Sample two was made with 2H/13C glucose, 99%D2O and 15N4Cl in standard M9
but with addition of uniform gamma protonated 13C/2H precursors 30 minutes
prior to induction as described by Goto et al, JBNMR.

[U-2H; NA-Ile,Leu,Val metthyl; U-13C; U-15N]

The data from this study were used to determine the structure of the NUDIX
domain of yeast Dcp2 residues 100-245 as a C-terminal fusion to the B1 domain
of protein G

Structural Analysis of the NUDIX domain of budding yeast Dcp2 , residues 100-245