NMR assignment of Human Ubiquitin Conjugating Enzyme (Ubc7)

Referenced 1H to water,
used PSSI to reference 13C and 15N
PSSI is used to get the chemical shift offsets for the indirect referencing

NMR assignment of human ubiquitin conjugating enzyme Ubc7.

Cook, W.J., Martin, P.D., Edwards, B.F.P., Yamazaki, R.K., and Chau, V.
(1997) Biochemistry, 36, 1621-1627.

Crystal structure of a class I ubiquitin conjugating enzyme (Ubc7) from Saccharomyces cerevisiae at 2.9 angstroms resolution.

1H, 13C, and 15N chemical shift resonance assignments for Human Ubc7

1mM, uniformly 13C, 15N labeled Ubc7, 50mM Tris-HCl buffer, pH 7.5, 2mM TCEP, 0.05% NaN3, 10% D20 (v/v)

1mM, uniformly 2H, 13C, 15N labeled Ubc7, 50mM Tris-HCl buffer, pH 7.5, 2mM TCEP, 0.05% NaN3, 10% D20 (v/v)

1H, 13C, and 15N chemical shift assignments for human ubiquitin-conjugating
enzyme, Ubc7

1H, 13C, and 15N Chemical Shift Assignments for Human Ubc7

Near complete 1H, 13C, and 15N chemical shift assignments (missing M1,A2,G3,P20,P68 and aromatic sidechain resonances)

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax