1H, 15N and 13C chemical shift assignment of the Pleckstrin Homology domain of
the Protein kinase B (PKB/Akt)

Pleckstrin Homology domain of the human Protein Kinase B beta

Several unassigned signals were observed that may correspond to local
conformational exchange on an intermediate timescale arising from the bridge
isomerization.

1H, 15N and 13C chemical shift assignments of the Pleckstrin Homology domain of the Human Protein Kinase B (PKB/Akt).

A novel serine/threonine protein kinase (termed rac-PK) has recently been
identified and cloned from cDNA libraries derived from the human cell lines
MCF-7 and WI38. A second form of this protein kinase, termed rac protein kinase
beta, has been identified from cDNAs derived from the same cell lines. These
two closely related forms show 90% homology, although the beta form with a
predicted Mr 60,200 has a carboxyl terminal extension of 40 amino acids in
comparison to the alpha form. This extension has a high serine content with 11
serine residues in the last 30 amino acids. The beta form of the protein has
been shown by both in vitro translation and bacterial expression to be
approximately 5000 Da larger than the alpha form. rac protein kinase beta is
encoded by a 3.4-kb transcript and the alpha form is encoded by a 3.2-kb mRNA.
Using gene-specific probes both transcripts were detected in all cell types
analyzed, although levels of expression were different for the two forms. The
catalytic domain of rac protein kinase beta shows a high degree of homology to
both the protein kinase C and cyclic AMP-dependent protein kinase families, and
hence rac protein kinases appear to represent a new subfamily of the second
messenger serine/threonine protein kinases.

Jones PF, Jakubowicz T, Hemmings BA.
Molecular cloning of a second form of rac protein kinase.
Cell Regul. 1991 Dec;2(12):1001-9.

Molecular cloning of a second form of rac protein kinase.

Pons, J.L., Malliavin, T.E. & Delsuc, M.A. (1996)
J. Biomol. NMR 8, 445-452.

This sequence concerns the PH domain of the human PKB-beta (AKT2).

Solution Structure Of The Pleckstrin Homology Domain Of Human Protein Kinase B Beta (PkbAKT)

RAC protein kinase beta [Rattus norvegicus]

protein serine/threonine kinase [Homo sapiens]

Thymoma viral proto-oncogene 2 [Mus musculus]

RAC-beta serine/threonine-protein kinase [Mus musculus]

RAC-beta serine/threonine-protein kinase [Papio anubis]

RAC-beta serine/threonine-protein kinase [Bos taurus]

RAC-beta serine/threonine-protein kinase [Sus scrofa]

RAC-beta serine/threonine-protein kinase [Macaca mulatta]

RecName: Full=RAC-beta serine/threonine-protein kinase; AltName: Full=Protein kinase Akt-2; AltName: Full=Protein kinase B beta

TPA: v-akt murine thymoma viral oncogene homolog 2 [Bos taurus]

A cryogenic probe equipped the 500 MHz spectrometer.