Assignment of backbone 1H, 13C and 15N resonances of ParG, a protein required
for active partition of bacterial plasmids

required for active partition of bacterial plasmids

ParG, a protein required for active partition of bacterial plasmids, has a dimeric ribbon-helix-helix structure.

The segregational stability of bacterial, low-copy-number plasmids is promoted
primarily by active partition. The plasmid-specified components of the
prototypical P1 plasmid partition system consist of two proteins, ParA (44.3
kDa) and ParB (38.5 kDa), which, in conjunction with integration host factor,
form a nucleoprotein complex at the plasmid partition site, parS. This complex
is the probable substrate for the directed temporal and spatial intracellular
movement of plasmids before cell division. The genetic organization of the
partition cassette of the multidrug resistance plasmid TP228 differs markedly
from that of the P1 paradigm. The TP228 system includes a novel member (ParF;
22.0 kDa) of the ParA superfamily of ATPases, of which the P1 ParA protein is
the archetype. However, the ParF protein and its immediate relatives form a
discrete subgroup of the ParA superfamily, which evolutionarily is more related
to the MinD subgroup of cell division proteins than to ParA of P1. The TP228
and P1 partition modules differ further in that the former does not include a
parB homologue, but does specify a protein (ParG; 8.6 kDa) unrelated to ParB.
Homologues of the parF gene are widely disseminated on eubacterial genomes,
suggesting that ParF-mediated partition may be a common mechanism by which
plasmid segregational stability is achieved.

Hayes F.
The partition system of multidrug resistance plasmid TP228 includes a novel
protein that epitomizes an evolutionarily distinct subgroup of the ParA
superfamily.
Mol Microbiol. 2000 Aug;37(3):528-41.

The partition system of multidrug resistance plasmid TP228 includes a novel protein that epitomizes an evolutionarily distinct subgroup of the ParA superfamily.

plasmid partition protein ParG [Salmonella enterica subsp. enterica serovar Newport]

plasmid partitioning protein ParG [Escherichia coli]

partitioning protein ParB [Salmonella enterica subsp. enterica serovar Dublin]

hypothetical protein Sententeri_18255 [Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853]

Salmonella enterica subsp. enterica serovar Newport

ARIA protocol (Nilges, M. et al., (1997) J. Mol. Biol. 269, 408-422) was used to deal with ambiguous distance restraints and for some NOE assignments.