Enabling Site-Specific NMR Investigations of Therapeutic Fab
using a Cell-Free Based Isotopic Labeling Approach:
Application to anti-LAMP1 Fab.

Enabling site-specific NMR investigations of therapeutic Fab using a cell-free based isotopic labeling approach: application to anti-LAMP1 Fab.

The sequence of the Fab fragment is composed with two amino acids chains:
the light chain and the heavy chain. The two chain are linked thanks to a
disulfide bond between two cysteines at their C-terminal.

The sequence of the Fab fragment is composed with two amino acids chains:
the light chain and the heavy chain. The two chain are linked thanks to
a disulfide bond between two cysteines at their C-terminal.

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Backbone NMR assignment of the anti-LAMP1 Fab fragment labelled and produced in a cell-free system.

Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale (IBS)

U-[1H,13C,15N] labelled Anti-LAMP1 Fab fragment