1H and 15N backbone resonance assignments of the D10N,P146A variant of beta-phosphoglucomutase (trans K145-A146 peptide bond) in a Mg-bound complex with fructose 1,6-bisphosphate

The trans-A Mg-bound bPGM-D10N,P146A:F16BP complex has a trans K145-A146 peptide bond
trans-A Mg-bound bPGM-D10N,P146A:F16BP complex

The trans-A Mg-bound bPGM-D10N,P146A:F16BP complex describes a near-attack conformation containing a bound cellular phosphorylating agent

[(2R,3S,4S,5R)-2,3,4-trihydroxy-5-(phosphonooxymethyl)oxolan-2-yl]methyl dihydrogen phosphate

Peri active site catalysis of proline isomerisation is the molecular basis of allomorphy in β-phosphoglucomutase.

The trans-A Mg-bound bPGM-D10N,P146A:F16BP complex has a population of 28 % in the NMR sample and chemical shift assignments are described in this entry
The trans-A Mg-bound bPGM-D10N,P146A:F16BP:MgT complex has a population of 72 % in the NMR sample and is described in another entry
These complexes are in slow conformational exchange

D10N (residue assigned as the general acid-base)
P146A

Wild-type beta-phosphoglucomutase catalyses the reversible interconversion of beta-glucose 1-phosphate and glucose 6-phosphate via a beta-glucose 1,6-bisphosphate reaction intermediate

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1H, 15N and 13C backbone resonance assignments of the D10N variant of beta-phosphoglucomutase (cis K145-P146 peptide bond) in a MgII-bound complex with beta-glucose 1,6-bisphosphate

1H, 15N and 13C backbone resonance assignments of the P146A variant of beta-phosphoglucomutase in its substrate-free form

1H, 15N and 13C backbone resonance assignments of the D10N variant of beta-phosphoglucomutase (cis K145-P146 peptide bond) in a Mg-bound complex with fructose 1,6-bisphosphate

1H, 15N and 13C backbone resonance assignments of the D10N,P146A variant of beta-phosphoglucomutase (trans K145-A146 peptide bond) in a Mg-bound complex with fructose 1,6-bisphosphate and an additional Mg ion bound in the active site

1H, 15N and 13C backbone resonance assignments of the D10N,P146A variant of beta-phosphoglucomutase (trans K145-A146 peptide bond) in a Mg-bound complex with beta-glucose 1,6-bisphosphate and an additional Mg ion bound in the active site

1H, 15N and 13C backbone resonance assignments of the D10N,P146A variant of beta-phosphoglucomutase (cis K145-A146 peptide bond) in a Mg-bound complex with beta-glucose 1,6-bisphosphate

1H, 15N and 13C backbone resonance assignments of the D10N,P146A variant of beta-phosphoglucomutase (trans K145-A146 peptide bond) in a Mg-bound complex with beta-glucose 1,6-bisphosphate

Crystal structure of the D10N variant of beta-phosphoglucomutase from Lactococcus lactis trapped with native beta-glucose 1,6-bisphosphate intermediate to 1.9 A resolution

Crystal structure of the P146A variant of beta-phosphoglucomutase from Lactococcus lactis in its substrate-free form