NT8-13 & ML314 bound enNTS1DM4 with G alpha iq & PIP2

2 ligands, NT8-13 & ML314; 1 transducer protein G alpha iq; 1 or more PIP2 molecules
NT8-13 & ML314 bound enNTS1DM4 with Gaiq & PIP2

Multiple peaks (in slow exchange) were observed for residues M204, M250, M330 & M352
NT8-13 & ML314 bound enNTS1DM4 13CH3-Met with G alpha iq & PIP2

Arrestin-biased agonist for NTS1 and positive allosteric modulator for NT binding to NTS1. CAS No. 1448895-09-7

Stabilization of pre-existing neurotensin receptor conformational states by β-arrestin-1 and the biased allosteric modulator ML314.

Phosphatidylinositol-4,5-bisphosphate (PIP2)

Thermostabilized rat neurotensin receptor 1 variant starts with the first T (T43) and ends with T416 (truncated).
The protein contains a C-terminal Avi-tag and remnants of a 3C-protease cleavage tag (ASGLNDIFEAQKIEWHEGSGLEVLFQ).
The N-terminal four residues (GPGS) are remnants of a 3C-protease cleavage site and a short, flexible GS sequence.

The first two residues (GLY1 & PRO2) are a remnant from HRV 3C cleavage, followed by a short linker (GLY3 & SER4);
G alpha iq starts at GLY5.

G alpha iq is a chimera of G alpha i1 with the six C-terminal residues from G alpha q (KEYNLV)

Arrestin-biased agonist for NTS1 and allosteric modulator of NT binding to NTS1

NT8-13 & ML314 bound enNTS1DM4 with PIP2

NT8-13 bound enNTS1DM4 with beta-Arrestin1-3A

NT8-13 bound enNTS1DM4 with beta-Arrestin1-3A & PIP2

ML314 bound enNTS1DM4 with beta-Arrestin1-3A & PIP2

NT8-13 & ML314 bound enNTS1DM4 with beta-Arrestin-1-3A & PIP2

buffer contained 50mM Potassium phosphate, 100mM NaCl, 20uM DSS, >0.05% DDM