Backbone and side-chain chemical shift assignments of the MazE9 antitoxin in Mycobacterium tuberculosis

Assigned chemical shifts for the major conformer of MazE9

Assigned chemical shifts for the minor conformer of MazE9

Insights into the solution structure and transcriptional regulation of the MazE9 antitoxin in Mycobacterium tuberculosis.

toxin antitoxin system, Mycobacterium tuberculosis

Numbering starts from 4, to be consistent with the numbering scheme of the other truncation and single site mutants used in this study.

It is also involved in auto-regulation of the operon encoding for this toxin-antitoxin cassette in Mtb.

MazE9 is an antitoxin, that neutralizes the activity of the toxin MazF9.

MazE9 is the antitoxin component of the MazEF9 toxin-antitoxin system in Mycobacterium tuberculosis belonging to the genomic locus Rv2801A.
It also carries an alternative name, mazE-mt1.
Here, the chemical shift assignments of the 76 residue long MazE9 antitoxin has been provided.

C terminal domain (CTD) of MazE9 antitoxin

N terminal domain of the MazE9 (nMazE9) antitoxin

After purification, buffer exchange in phosphate buffer prepared in D2O (pH 7.0) performed.