Bacillus subtilis SepF protein assembly (wild type)

Structural Insights into the Interaction between Bacillus subtilis SepF Assembly and FtsZ by Solid-State NMR Spectroscopy.

Residues 1-151 are shown in the upper box.
The samples detected by solid-state NMR experiments are SepF truncations of 57-151.

Through a series of solid-state NMR experiments, we assigned chemical shifts of the
SepF truncation (57-151) of the protein assembly associated with bacterial division.
72 residues of 95 residues (~76%) of SepF 57-151 were assigned in its assembly
including all the carbon and nitrogen atoms (N, CO, CA, CB, CG, CD, CE, NE).

Innovation Academy for Precision Measurement Science and Technology, CAS

[U-13C, 15N]-labeled SepF 57-151 are prepared for the 2D and 3D assignment
experiments including NCA, NcaCX, NCACX, NCOCX, CONCA .etc