1H, 13C and 15N resonance assignments of diglycosylated TSR3 domain of human Thrombospondin-1

O-Fucosylation stabilizes the TSR3 motif in Thrombospondin-1 by interacting with nearby amino acids and protecting a disulfide bond.

glycosylation, extracellular matrix, O-fucose, thrombospondin type 1 repeats, protein folding

Residues 9-67 correspond to segment 490-548 of the native thrombospondin-1.
Residues 1-8 and 68-75 represent non-native expression and purification tags.
Residue 42 represents a naturally occuring sequence variant p.Thr523Ala.

Department of Biochemistry and Molecular Biology

Chemical shifts of non-glycosylated TSR3