Backbone 1H, 13C, and 15N Chemical Shift Assignments for Transforming Growth Factor Beta Receptor 1 (TbRI) as bound to Domain 2 of Heligmosomoides polygyrus protein Transforming Growth Factor Beta Mimic 1 (TGM-1 D2)

If the chemical shift value for an atom is derived from multiple experiments,
the error is set as the standard deviation of the set of chemical shift values.
If the chemical shift value is derived from a single experiment, for 1H the
error is set as 0.05, for 13C the error is set as 0.2, and for 15N the error
is set as 0.2.

TbRI bound to TGM-1 D2 Chemical Shift Assignment

Convergent evolution of a parasite-encoded complement control protein-scaffold to mimic binding of mammalian TGF-β to its receptors, TβRI and TβRII.

TGF-B, TGF-B mimic, TGM, H. polygyrus, parasite

molecular mimicry, regulatory T-cell, immune suppression

nuclear magnetic resonance, isothermal titration calorimetry, surface plasmon resonance

Residues 6 and 24, residues 8 and 11, residues 18 and 41,
residues 56 and 70, and residues 71 and 76 are disulfide bonded,
with residues numbered starting from 1.

Residues 31-115 of the human TGF-B type I receptor

Residues 4 and 67 are disulfide linked, as are residues 50 and 80.
The first two residues are part of a KpNI cut site

TGF-beta receptor type-1 isoform 1 precursor

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Hinck Laboratory, University of Pittsburgh School of Medicine Department of Molecular Biophysics and Structural Biology

15N 13C TbRI with an excess of unlabeled TGM-1 D2

Transforming Growth Factor Beta Receptor 1 (TbRI)

25mM HEPES, 50mM NaCl, 0.02% azide pH 6.0