Phosphorylated phospholamban bound to SERCA in oriented bicelles (calcium-bound E1 state)

Phosphorylated phospholamban bound to SERCA (calcium-bound E1 state)

Anisotropic chemical shifts and dipolar couplings (Hz) measured using a PISEMA-like experiment (SE-SAMPI4).

(2S)-2-amino-3-phosphonooxy-propanoic acid

Structural basis for allosteric control of the SERCA-Phospholamban membrane complex by Ca2+ and phosphorylation.

pS16-PLN-AFA with SERCA (E1) 15N-1H Dipolar Couplings

Monomeric cysteine-null mutant (C36A/C41F/C46A).
N-terminal Ala0 left over from cleavage of MBP fusion protein.
Phosphorylated at Ser16 by protein kinase A.

Orientated 15N chemical shift correlations with 15N-1H dipolar couplings.

Monomeric phospholamban in oriented bicelles

Monomeric phosphorylated phospholamban in oriented bicelles

Phospholamban bound to SERCA in oriented bicelles (calcium-free E2 state)

Phospholamban bound to SERCA in oriented bicelles (calcium-bound E1 state)

Phosphorylated phospholamban bound to SERCA in oriented bicelles (calcium-free E2 state)

Monomeric Ser16-phosphorylated phospholamban in complex with SERCA in oriented bicelles (calcium-bound E1 state)

Ser16-phosphorylated phospholamban (pPLN-AFA)

1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)

1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC)

1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC)

1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-diethylenetriaminepentaacetic acid (PE-DTPA)

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (SERCA1a)

Monomeric phospholamban reconstituted with SERCA in bicelles.