Titration of C8-AcpP with the E. coli thioesterase 1 TesA

The C8 pantetheine probe was loaded overnight in a chemoenzymatic reaction
to create a stable octanoic pantetheine probe.

These were adapted from previous Burkart work in BMRB entry 27874.
Which was itself assigned based on original work in BMRB entry 3.

Elucidation of transient protein-protein interactions within carrier protein-dependent biosynthesis.

2D 1H-15N HSQC AcpP TesA titration saturated

2D 1H-15N HSQC AcpP TesA titration zero point

Titration of AcpP with the E. coli 3-oxoacyl-[acyl-carrier-protein] reductase FabG

Titration of C8-AcpP with the E. coli Enoyl-[acyl-carrier-protein] reductase FabI

The titration of AcpP with TesA, saturated sample.
10mM potassium phosphate pH 7.4 buffer with 0.5mM TCEP and 0.1% NaN3.

The titration of AcpP with TesA, zero point sample.
10mM potassium phosphate pH 7.4 buffer with 0.5mM TCEP and 0.1% NaN3.

10mM potassium phosphate pH 7.4 buffer with 0.5mM TCEP and 0.1% NaN3.