Backbone 13C, and 15N Chemical Shift Assignments for ChiZ N-terminal Domain

Sequence-Dependent Correlated Segments in the Intrinsically Disordered Region of ChiZ.

ChiZ residues 1 to 64 plus first 3 residues (SNA) left from TEV protease cleavage site.
Residues 1-64 correspond to the cytoplasmatic soluble domain of ChiZ membrane protein.

Involved in Mycobacterium tuberculosis cell division

Bruker pulse sequence used: fhsqccxf3gpph.
Spin lock times used: 10 ms, 15 ms, 20 ms, 25 ms, 30 ms, 40 ms, 50 ms, 75 ms, and 100 ms. relaxation delay of 3 s was used.

Bruker pulse sequence used hsqcnoef3gpsi.

Bruker pulse sequence used hsqct1etf3gpsi with the following delays: 10 ms, 62.5 ms, 125 ms, 250 ms, 500 ms, 750 ms, 1000 ms, and 1500 ms.
Relaxation delay between scans: 6 s

Bruker pulse sequence used hsqct2etf3gpsi with the following delays: 5 ms, 30 ms, 62.5 ms, 125 ms, 187.5 ms, 250 ms, 312.5 ms and 375 ms.
Relaxation delay between scans: 4 s

13C-15N uniformly label ChiZ1-64 sample concentration was 1 mM at pH 7.0