1H sequential assignment and solution secondary structure of mouse MIP-3a
chemokine

NMR solution structure of murine CCL20/MIP-3alpha, a chemokine that specifically chemoattracts immature dendritic cells and lymphocytes through its highly specific interaction with the beta-chemokine receptor CCR6.

We have cloned the murine CCR6 receptor and its ligand, the beta-chemokine
mMIP-3alpha. Calcium mobilization assays performed with mCCR6 transfectants
showed significant responses upon addition of mMIP-3alpha. Murine MIP-3alpha
RNA is expressed in thymus, small intestine and colon, whereas mCCR6 RNA is
expressed in spleen and lymph nodes. RT-PCR analysis of FACS-sorted lymphoid
and antigen presenting cell subsets showed mCCR6 expression mainly in B cells,
CD8- splenic dendritic cells and CD4+ T cells. The cloning and functional
characterization of the mCCR6 and mMIP-3alpha will allow the study of the role
of these proteins in mouse models of inflammation and immunity.

Molecular cloning, functional characterization and mRNA expression analysis of the murine chemokine receptor CCR6 and its specific ligand MIP-3alpha.

Chemokines are involved in the control of dendritic cell (DC) trafficking, which
is critical for the immune response. We have generated DC from human umbilical
cord blood CD34+ progenitors cultured with granulocyte-macrophage
colony-stimulating factor, tumor necrosis factor alpha (TNF-alpha), and stem
cell factor. Using an anti-CCR6 monoclonal antibody, we observed that these
cells showed maximum expression of this beta-chemokine receptor when they were
immature, as determined by their relatively low expression of several DC
maturation markers such as CD1a, CD11c, CD14, CD40, CD80, and CD83. Immature DC
responded strongly to macrophage inflammatory protein-3alpha (MIP-3alpha), the
CCR6 ligand, in migration and calcium mobilization assays. CCR6 expression
decreased in parallel with the DC maturation induced by prolonged TNF-alphaq
treatments. Interleukin-4 was also able to decrease CCR6 protein levels. Our
findings suggest that the MIP-3alpha/CCR6 interaction plays an important role
in the trafficking of immature DC to chemokine production sites such as injured
or inflamed peripheral tissues, where DC undergo maturation on contact with
antigens.

Down-regulation of the beta-chemokine receptor CCR6 in dendritic cells mediated by TNF-alpha and IL-4.

Nmr Solution Structure Of Murine Ccl20MIP-3a Chemokine

CC chemokine ST38 precursor [Mus musculus]

macrophage inflammatory protein 3 alpha [Mus musculus]

Chemokine (C-C motif) ligand 20 [Mus musculus]

chemokine (C-C motif) ligand 20, isoform CRA_a [Mus musculus]

chemokine (C-C motif) ligand 20, isoform CRA_b, partial [Mus musculus]

C-C motif chemokine 20 isoform 2 precursor [Mus musculus]

C-C motif chemokine 20 isoform 1 precursor [Mus musculus]

PREDICTED: C-C motif chemokine 20 isoform X1 [Mus musculus]

PREDICTED: C-C motif chemokine 20 isoform X2 [Mus musculus]

RecName: Full=C-C motif chemokine 20; AltName: Full=Beta-chemokine exodus-1; AltName: Full=CC chemokine LARC; AltName: Full=CC

TORSIONAL ANGLE DYNAMICS AND MOLECULAR DYNAMICS