Sequential NMR assignment of the Ras-binding domain of Byr2

The RAS-binding domain of the protein kinase Byr2 is studied

Sequential assignments were obtained
for all amino acids with the exception
of P83, S84, R90 and P110.
In the region P83-E93 signals in all
spectra were generally very weak and
only rudimentary side chain assignments
could be obtained.

Huber, F., Gronwald, W., Wohlgemuth, S., Herrmann, C., Geyer, M., Wittinghofer, A.,
and Kalbitzer, H.R., "Letter to the Editor: Sequential NMR Assignment of the
RAS-binding Domain of Byr2," J. Biomol. NMR 16, 355-356 (2000).

Schizosaccharomyces pombe contains a single gene, ras1, which is a homolog of
the mammalian RAS genes. ras1 is required for conjugation, sporulation, and
normal cell shape. ras1 has been previously identified as ste5. We report here
a gene we call byr2 that can encode a predicted protein kinase and can
partially suppress defects in ras1 mutants. ras1 mutant strains expressing high
levels of byr2 can sporulate competently but are still defective in conjugation
and abnormally round. byr2 mutants are viable and have normal shape but are
absolutely defective in conjugation and sporulation. byr2 is probably identical
to ste8. In many respects, byr2 resembles the byr1 gene, another suppressor of
the ras1 mutation, which has been identified previously as ste1. Our data
indicate that if ras1, byr2, and byr1 act along the same pathway, then the site
of action for byr2 is between the sites for ras1 and byr1.

Mol Cell Biol 1991 Jul;11(7):3554-63

byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial
suppression of the ras1 mutant phenotype.

Wang Y, Xu HP, Riggs M, Rodgers L, Wigler M

byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial suppression of the ras1 mutant phenotype.

Neidig, K.-P., Geyer, M., Gorler, A., Antz, C.,
Saffrich, R., Beneicke, W. and Kalbitzer, H. R.
(1995) J. Biomol. NMR, 6, 255-270.

Solution Structure Of The Ras-Binding Domain Of The Protein Kinase Byr2 From Schizosaccharomyces Pombe

Crystal Structure Of Ras-Bry2rbd Complex

protein kinase [Schizosaccharomyces pombe]

MAP kinase kinase kinase Byr2 [Schizosaccharomyces pombe]

MAP kinase kinase kinase Byr2 [Schizosaccharomyces pombe 972h-]

RecName: Full=Protein kinase byr2; AltName: Full=MAPK kinase kinase; Short=MAPKKK; AltName: Full=Protein kinase ste8

2D NOESY and TOCSY spectra measured in H2O