Solution Structure and Dynamics of a De Novo Designed Three-helix Bundle Protein

Walsh, S.T.R., Cheng, H., Bryson, J.W., Roder, H., and DeGrado, W.F.,
"Solution Structure and Dynamics of a De Novo Designed Three-helix Bundle
Protein," Proc. Natl. Acad. Sci. USA 96, 5486-5491 (1999).

Solution Structure Of A De Novo Designed Single Chain Three- Helix Bundle (A3d)

a3D was cloned into pET-16b using the Nco-I and HindIII restriction sites.
The protein was produced in E. coli strain BL21(DE3).