NMR solution structure of the N-terminal RRM and flanking linker regions of Polypyrimidine tract binding protein 1 using the CYANA CONSENSUS method.

15N T2-relaxation-dispersion CPMG-15NHSQC

15N T2-relaxation-dispersion-CPMG-15NHSQC

cross-correlated DD/CSA relaxation-15NHSQC

separate tube (no insert) similar to the experimental sample tube

A transient α-helix in the N-terminal RNA recognition motif of polypyrimidine tract binding protein senses RNA secondary structure.

N-terminal domain of Polypyrimidine-tract binding protein is a dynamic folding platform for adaptive RNA recognition

Heterogeneous nuclear ribonucleoprotein I

gene for construct inserted between the NdeI and Xho1 sites

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STRUCTURE WAS CALCULATED USING CONSENSUS METHOD
(BUCHNER L AND GUENTERT P 2015 STRUCTURE 23, 425-434).
20 INDEPENDENT STRUCTURE CALCULATIONS OF 100 CONFORMERS
WERE PERFORMED AND A SET OF CONSTRAINTS CONSISTENT AMONG
60% OF THE ENSEMBLES WAS COMBINED FOR A FINAL CONSENSUS
CALCULATION TO GENERATE THIS ENSEMBLE
THE PROTEIN CONTAINS A DYNAMIC REGION WITH RESIDUAL STRUCTURE.
DUE TO THE AMBIGUOUS NATURE OF THE CONSTRAINTS AND THE DYNAMIC
REGION NO ENERGY REFINEMENT WAS PERFORMED

Z-axis gradient triple resonance cryoprobe

Z-axis gradient triple resonance RT probe

0.8 mM [U-15N]-99% Polypyrimidine-tract binding protein N-terminal RNA recognition motif,
10 mM NaH2PO4/NaOH buffer, 20 mM sodium chloride, 90% H2O/10% D2O

0.8 mM [U-13C,15N]-99% Polypyrimidine-tract binding protein N-terminal RNA recognition motif,
10 mM NaH2PO4/NaOH buffer, 20 mM sodium chloride, 90% H2O/10% D2O

0.8 mM [U-15N]-99% Polypyrimidine-tract binding protein N-terminal RNA recognition motif,
10 mM NaH2PO4/NaOH buffer, 20 mM sodium chloride, 0.8 mM [U-13C,15N]-99% UCUUU-SL-RNA, 90% H2O/10% D2O

Polypyrimidine-tract binding protein N-terminal RNA recognition motif

RNA BINDING PROTEIN Alternative splicing repressor IRES trans-acting factor RNA recognition motif Dynamic structure