Zn(II) form of shortened metallothionein from Pseudomonas fluorescens Q2-87 (residues 1-52)

C' shifts from the HNCO otherwise shifts were extracted from the HSQC spectra

Metallomics : integrated biometal science
A histidine-rich Pseudomonas metallothionein with a disordered tail displays higher binding capacity for cadmium than zinc.

CRYO TCI inverse triple-resonance (1H; 13C; 15N) with a cold 13C-Channel and an actively shielded z-gradient coil

CRYO TXI inverse triple-resonance (1H; 13C; 15N) with an actively shielded z-gradient coil

0.5 mM [U-13C; U-15N] metallothionein, 1.5 mM ZINC ION,
50 mM [U-2H] TRIS, 50 mM sodium chloride, 90% H2O/10% D2O

Schwieters, Kuszewski, Tjandra and Clore