Solution structure of BOLA1 from Homo sapiens

Mitochondrial Bol1 and Bol3 function as assembly factors for specific iron-sulfur proteins.

GIDPFT sequence at the N-terminus is added by cloning.
The mitochondrial-targeting sequence (MLSGRLVLGLVSMAGRVCLC) has been removed.

1 mM [U-100% 15N] mitochondrial BOLA1 protein, 50 mM potassium phosphate, 5 mM DTT, 90% H2O/10% D2O

1 mM [U-100% 13C; U-100% 15N] mitochondrial BOLA1 protein, 50 mM potassium phosphate, 5 mM DTT, 90% H2O/10% D2O

Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman