BIVALENT INTERACTIONS OF PIN1 WITH THE C-TERMINAL TAIL OF PKC

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (E.C.5.2.1.8), PROTEIN KINASE C BETA TYPE (E.C.2.7.11.13)

(2S)-2-amino-3-phosphonooxy-propanoic acid

(2S,3R)-2-amino-3-phosphonooxy-butanoic acid

3D [F1] 13C, 15N-FILTERED NOESY- 13Caro-HSQC

3D [F1] 13C, 15N-FILTERED NOESY- 13Cali-HSQC

Referencing external sample: 2mM sucrose+0.5 mM DSS+ 2mM NaN3
(90%H2O/10%D2O)

Sequestration Without Isomerization: Non-Catalytic Role of Pin1 in PKC Regulation

Peptidyl-prolyl cis-trans isomerase Pin1

1.05 mM [U-13C; U-15N] PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1,
0.9 mM The C-terminal tail of PKC, 10 mM [U-100% 2H] imidazole,
100 mM potassium chloride, 1 mM TCEP, 0.02 % w/v sodium azide, 92% H2O/8% D2O

1.05 mM [U-13C; U-15N] PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1,
0.9 mM The C-terminal tail of PKC, 10 mM [U-100% 2H] imidazole,
100 mM potassium chloride, 1 mM TCEP, 0.02 % w/v sodium azide, 100% D2O

1 mM [U-13C; U-15N] PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1,
1.3 mM The C-terminal tail of PKC, 10 mM [U-100% 2H] imidazole,
100 mM potassium chloride, 1 mM TCEP, 0.02 % w/v sodium azide, 92% H2O/8% D2O

0.8 mM [U-13C; U-15N] PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1,
5.5 mM The C-terminal tail of PKC, 10 mM [U-100% 2H] imidazole,
100 mM potassium chloride, 1 mM TCEP, 0.02 % w/v sodium azide, 92% H2O/8% D2O

0.5 mM [U-13C; U-15N] PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1,
1.8 mM The C-terminal tail of PKC, 10 mM [U-100% 2H] imidazole,
100 mM potassium chloride, 1 mM TCEP, 0.02 % w/v sodium azide, 92% H2O/8% D2O

0.5 mM [U-13C; U-15N] PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1,
2.5 mM The C-terminal tail of PKC, 10 mM [U-100% 2H] imidazole,
100 mM potassium chloride, 1 mM TCEP, 0.02 % w/v sodium azide, 92% H2O/8% D2O

1 mM [U-13C; U-15N] PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1,
1.5 mM The C-terminal tail of PKC, 10 mM [U-100% 2H] imidazole,
100 mM potassium chloride, 1 mM TCEP, 0.02 % w/v sodium azide, 92% H2O/8% D2O

1.3 mM [U-13C; U-15N] PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1,
1 mM The C-terminal tail of PKC, 10 mM [U-100% 2H] imidazole,
100 mM potassium chloride, 1 mM TCEP, 0.02 % w/v sodium azide, 92% H2O/8% D2O

0.8 mM [U-13C; U-15N] PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1,
1.3 mM The C-terminal tail of PKC, 10 mM [U-100% 2H] imidazole,
100 mM potassium chloride, 1 mM TCEP, 0.02 % w/v sodium azide, 92% H2O/8% D2O

PEPTIDYL-PROLYL CIS-TRANS ISOMERASE NIMA-INTERACTING 1

Schwieters, Kuszewski, Tjandra and Clore

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax