Solution structures of full-length K-RAS bound to GDP

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0.7-0.9 mM [U-100% 15N] GTPase KRas, 20 mM [U-2H] MES, 100 mM potassium chloride,
50 mM sodium chloride, 2 mM MgCl2, 1 mM [U-2H] TCEP, 7 mM [U-2H] D2O,
0.05 % sodium azide, 90% H2O/10% D2O

0.7-0.9 mM [U-13C; U-15N] GTPase KRas, 20 mM [U-2H] MES, 100 mM potassium chloride,
50 mM sodium chloride, 2 mM MgCl2, 1 mM [U-2H] TCEP, 7 mM [U-2H] D2O,
0.05 % sodium azide, 90% H2O/10% D2O

Lee W, Petit CM, Cornilescu G, Stark JL, Markley JL.

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax