Solution structure of the TRIM21 B-box2 (B2)

E3 ubiquitin-protein ligase TRIM21 (E.C.6.3.2.-)

All chemical shifts from 15-N and 13-C correlated experiments

Solution NMR structure of the TRIM21 B-box2 and identification of residues involved in its interaction with the RING domain.

52 kDa ribonucleoprotein autoantigen Ro/SS-A

450 uM [U-13C; U-15N] TRIM21 Bbox2, 50 mM TRIS, 150 mM sodium chloride, 10 mM beta-mercaptoethanol, 10 uM ZINC ION, 10 % glycerol, 90% H2O/10% D2O

450 uM [U-13C; U-15N] TRIM21 Bbox2, 50 mM deuterated TRIS, 150 mM sodium chloride, 10 mM beta-mercaptoethanol, 10 uM ZINC ION, 10 % glycerol, 90% H2O/10% D2O

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Brunger, Adams, Clore, Gros, Nilges and Read