Backbone resonances of the tSH2 domain in complex with the bidentate ITIM-ITSM peptide of PD-1. ITSM-bound state.

The term "ITSM-bound state" refers to the fact the assignments correspond to
the state in which the nSH2 of tSH2 is bound to the ITSM part of this peptide,
i.e. -EQTE(pY)ATIVFP.

tSH2 domain in complex with the bidentate ITIM-ITSM peptide of PD-1

(2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid

tandem-pep8-HN(COCA)CB (H[N[co[{CA|ca[C]}]]])

1H, 13C, 15N chemical shift assignments of SHP2 SH2 domains in complex with PD-1 immune-tyrosine motifs.

The tandem SH2 domain of SHP2 interacts with the bidentate ITIM-ITSM phosphopeptide creating a heterogeneous mixture of bound states.
While cSH2 is always bound to ITSM, nSH2 can be bound to ITIM or to ITSM, depending on the ratio of peptide to protein.
Here, the resonances of the state in which nSH2 is bound to ITSM are reported.

cSH2 domain of SHP2 in complex with the ITIM of PD-1

nSH2 domain of SHP2 in complex with the ITSM of PD-1

nSH2 domain of SHP2 in complex with the ITIM of PD-1

tSH2 domain in complex with the bidentate ITIM-ITSM peptide of PD-1, ITIM-bound state

The CCPN NMR assignment and data analysis application

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

Department of Biochemistry, Cambridge CB2 1GA, UK