side-chain methyl order parameters for apo CzrA L34A mutant at 25C-40C

Journal of the American Chemical Society
Functional role of solvent entropy and conformational entropy of metal binding in dynamically-driven allostery.

Methyl order parameter data recorded using the latest version of the experiments
by Tugarinov, Sprangers, Kay (J.Am.Chem.Soc. 129,1743). Slow-timescale dynamics
recorded using the latest version of the experiments by Korzhnev, Kay, et al.
(J.Am.Chem.Soc. 126, 3964).

Resonance assignments of CzrA in apo form

side-chain methyl order parameters, stereospecific resonance assignments, and relaxation rates for apo CzrA

side-chain methyl order parameters, stereospecific resonance assignments, and relaxation rates for Zn(II) CzrA

side-chain methyl order parameters for Zn(II)-bound CzrA L34A mutant at 25C-40C

Resonance assignments of CzrA in complex with Zinc ion

Resonance assignments of CzrA in complex with DNA

crystal structure of CzrA in Zn(II) form

Solution structure of CzrA in the DNA bound state

Soluction structure of CzrA in the Zn(II) state

Protein expressed in 100% D2O using 2H, 12C glucose as carbon source,
15N ammonium chloride as nitrogen source, with added 1H,13C-methyl-labeled
alanine and methionine plus alpha-ketobutyrate and alpha-ketoisovalerate.
alphaketoisovalerate is CDLM-7317 from Cambridge
Isotope Laboratories, with 2H and 12C except at one of the two methyls,
which is 1H and 13C. alpha-ketobutyrate is CIL CDLM-7318, with 2H and 12C
except at the methyl, which is 1H and 13C.

10 percent 13C for stereospecific assignments

[U-12C; U-15N; U-2H; Ala, Met, Leu, Val, Ile-d methyl 100%-1H; Ala, Met, Leu, Val, Ile-d methyl 100%-13C]

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax