1H, 13C and 15N assignment of C', CA, N and HN of intrinsically disordered alpha-synuclein near-phsysiological conditions

intrinsically disordered alpha-synuclein

assignment obtained for 13C-15N alpha-synuclein at 315 K with 5D APSY (HCA)CONCACON,
3D APSY HNCO, 2D 1H-15N HSQC, 2D CON experiments.
The NMR sample contained 1.0 mM uniformly 13C-15N labeled a-synuclein, 20 mM phosphate
buffer, 0.5 mM EDTA, 200 mM NaCl in 90% H2O/10% D2O at pH 6.5.

13C APSY-NMR for sequential assignment of intrinsically disordered proteins.

The 700 MHz NMR spectrometer was equipped with a TXO cryoprobe optimized for 13C direct detection

The NMR sample contained 1.0 mM uniformly 13C-15N labeled alpha-synuclein,
20 mM phosphate buffer, 0.5 mM EDTA, 200 mM NaCl in 90% H2O/10% D2O at pH 6.5