1H, 15N, 13C backbone resonance assignments of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2 in complex with oxidised flavin mononucleotide

TROSY offset: amide protons; amide nitrogens.
2H isotope effect: backbone CA, CB.

[(2R,3S,4S)-5-[7,8-dimethyl-2,4-bis(oxidanylidene)benzo[g]pteridin-10-yl]-2,3,4-tris(oxidanyl)pentyl] dihydrogen phosphate

1H, 15N and 13C backbone resonance assignments of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2.

Transverse relaxation optimised spectroscopy

Recombinant PETNR is expressed and purified as a tight non-covalently bound PETNR:FMNox complex

The FMN cofactor is oxidised in the PETNR:FMNox complex

pentaerythritol tetranitrate reductase (PETNR)

WILD-TYPE PENTAERYTHRITOL TETRANITRATE REDUCTASE CONTAINING A C-TERMINAL 8-HISTIDINE TAG FROM ENTEROBACTER CLOACAE

STRUCTURE OF OXIDISED PENTAERYTHRITOL TETRANITRATE REDUCTASE FROM ENTEROBACTER CLOACAE