C-terminal domain of merozoite surface protein 2

Lipid interactions modulate the structural and antigenic properties of the C-terminal domain of the malaria antigen merozoite surface protein 2.

First 50 residues are from the conserved C-terminal region of MSP2 and sequence
numbering of residues started with 172 which is based on numbering of mature
full-length of MSP2.

First 50 resides are conserved C-terminal MSP2 and this construct has two extra
residues (Leu, Glu) from the vector between the protein and His6-tag, giving a
total of 58 residues.

Interaction with lipid alters the antigenicity

MSP2 of the P. falciparum is a potential component of a malaria vaccine. The exact role of MSP2 is not clear but it has been shown to be essential for growth in vitro of asexual parasites.

Study the impact of lipid interaction on the C-terminal MSP2

Monash Institute of Pharmaceutical Science, Monash University

Sample used for recording 1H-15N HSQC spectra

Sample used for recording 1H-15N HSQC spectra in presence of DPC

Sample used for 3D heteronuclear triple resonance experiments(HNCACB, HN(CO)CACB and HNCA) to make resonance assignments

Sample used for 3D heteronuclear triple resonance experiments(HNCACB, HN(CO)CA) to make resonance assignments in the presence of DPC