PR-R7 from staphylocoagulase of S. aureus Newman D2 Tager 104 strain

Carbon and amide detect backbone assignment methods of a novel repeat protein from the staphylocoagulase in S. aureus.

combination of two C-terminal staphylocoagulase repeats

Center for Structural Biology, Vanderbilt University

Department of Pathology, Microbiology and Immunology, Vanderbilt University

350ul protein solution in 4mm tube with H2O inside 5mm tube filled with 200ul D2O

Use 4mm tube due to high ionic strength. D2O in outer tube regents N-D exchange on sample

Use 3mm tube due to high ionic strength.