1H, 15N, 13C backbone resonance assignments of human phosphoglycerate kinase in a transition state analogue complex with ADP, 3-phosphoglycerate and magnesium trifluoride

Transition state analogue of phosphoryl transfer

Systematic chem_shift offset:
TROSY offset: amide protons and amide nitrogens;
2H isotope effect: all 13C.

[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methyl phosphono hydrogen phosphate

(2R)-2-hydroxy-3-(phosphonooxy)propanoic acid

(2R)-2-hydroxy-3-phosphonooxy-propanoic acid

Transverse relaxation optimised spectroscopy

For comparison with 2WZB, PGK is numbered S1-L2-S3-N4-K5.....I416

PGK catalyses the formation of ATP from ADP and 1,3-bisphosphoglycerate in glycolysis

THE CATALYTICALLY ACTIVE FULLY CLOSED CONFORMATION OF HUMAN PHOSPHOGLYCERATE KINASE IN COMPLEX WITH ADP, 3PG AND MAGNESIUM TRIFLUORIDE