Backbone assignment of human 4E-BP1 (fragment 44 to 87) bound to mouse eIF4E

human 4E-BP1 (fragment 44 to 87) bound to mouse eIF4E

Origin xeasy file /nmr/data/Leger/4E-BP_assignment_deposition/complex_with_m4E/chemical_shifts.prot

1H, 13C, and 15N backbone chemical shift assignments of 4E-BP144-87 and 4E-BP144-87 bound to eIF4E.

The "GS" residues do not belong to the native mouse eIF4E sequence.
They come from the purification tag, which were cleaved following
TEV cleavage, but remained appended to the mouse eIF4E sequence.

Backbone assignment of 4E-BP1 bound to mouse eIF4E

human 4E-BP1 (fragment 44 to 87) (apo form)

20 mM NaH2PO4/Na2HPO4, pH 6.5, 50 mM NaCl, 2 mM DTT, 5% D2O