N-terminal domain of the E. coli DNA polymerase III delta subunit

E coli DNA polymerase III delta subunit (residues 1-140)

NMR resonance assignments for the N-terminal domain of the δ subunit of the E. coli γ clamp loader complex.

GSH are cloning artifacts. The native residues are M1-Q140

contributes to opening the beta-clamp for loading on DNA

his-tag was cleaved off with bovine thrombin

buffer:
20 mM sodium phosphate
100 mM NaCl
10 mM DTT
1 mM EDTA
0.05 % (w/v) sodium azide
0.33 % (v/v) Halt protease inhibitor cocktail
-diluted to 7.5% D2O

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax