1H, 15N, 13C backbone resonance assignments of human soluble catechol O-methyltransferase in complex with 3,5-dinitrocatechol and S-adenosyl-L-methionine

Complex of COMT with DNC, SAM and magnesium ion

(2S)-2-amino-4-[{[(2S,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl}(methyl)sulfonio]butanoate (non-preferred name)

1H, 15N, 13C backbone resonance assignments of human soluble catechol O-methyltransferase in complex with S-adenosyl-L-methionine and 3,5-dinitrocatechol.

transverse relaxation optimized spectroscopy

The human S-COMT construct used in this study has a 13 residue His-tag and
cloning sequence positioned at the N-terminus (MHHHHHHENLYFQG ), where G14 in
this construct corresponds with G2 in the canonical S-COMT sequence. This
construct also contains valine (rather than methionine) at the allelic
polymorphism position located at residue 108 of S-COMT.

methyltransferase enzyme, inactivates catecholamine neurotransmitters

1H, 15N, 13C backbone assignments for human catechol O-methyltransferase in complex with S-adenosyl-L-methionine and 3,5-dinitrocatechol

NMR spectrometer with TCI cryoprobe equipped with Z gradients

Sample was perdeuterated and then back exchanged.