Temperature Dependence of Molecular Interactions Involved in Defining Stability of Glutamine Binding Protein and Its Complex with L-Glutamine

Glutamine Binding Protein Open Ligand-Free Structure

glutamine transporter subunit [Escherichia coli str. K12 substr. W3110]

permease of periplasmic glutamine-binding protein [Escherichia coli O157:H7 str. Sakai]

glutamine ABC transporter substrate binding component [Escherichia coli SE11]

periplasmic glutamine-binding protein GlnH [Escherichia coli O26:H11 str. 11368]

periplasmic glutamine-binding protein GlnH [Escherichia coli O103:H2 str. 12009]

unnamed protein product [Escherichia coli K-12]

glutamine-binding periplasmic protein precursor [Salmonella enterica subsp. enterica serovar Typhi str. CT18]

glutamine-binding periplasmic protein [Escherichia coli LF82]

glnH, subunit of glutamine ABC transporter [Escherichia coli BL21(DE3)]

glutamine transporter subunit ; periplasmic binding component of ABC superfamily [Escherichia fergusonii ATCC 35469]

high-affinity periplasmic glutamine binding protein [Salmonella enterica subsp. enterica serovar Typhimurium]

glutamine transporter subunit [Escherichia coli str. K-12 substr. MG1655]

periplasmic glutamine-binding protein; permease [Escherichia coli O157:H7 str. EDL933]

glutamine high-affinity transporter [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]

periplasmic glutamine-binding protein; permease [Shigella flexneri 2a str. 301]

glutamine-binding periplasmic protein precursor [imported] - Salmonella enterica subsp. enterica serovar Typhi (strain CT18)

glutamine ABC transporter periplasmic protein [Escherichia coli O157:H7 str. Sakai]

glutamine-binding periplasmic protein [Salmonella enterica subsp. enterica serovar Typhi str. CT18]

glutamine ABC transporter substrate-binding protein [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]

glutamine ABC transporter substrate-binding protein [Shigella flexneri 2a str. 301]

RecName: Full=Glutamine-binding periplasmic protein; Short=GlnBP; Flags: Precursor

Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health

theta and phi are the Euler angles of the axially symmetric diffusion tensor axis in the frame of the original X-
ray coordinate file(3, 4)

200 ul of 20 uM protein solution were loaded in the sample cell, while the
injection syringe was loaded with 600 uM of the titrating L-gln.
Titration consisted of one 0.2 ul preliminary injection followed by 17
subsequent 1.2 ul injections.

Houtman, J. C. D., Brown, P. H., Bowden, B., Yamaguchi, H., Appella, E., Samelson, L. E., and Schuck, P.