Backbone 1H, 13C, and 15N chemical shift assignments for dimeric KaiB in complex with the CI domain of KaiC from the Thermosynechococcus elongatus BP-1 cyanobacterial species

Circadian rhythms. A protein fold switch joins the circadian oscillator to clock output in cyanobacteria.

Bruker 600 MHz AVANCE III spectrometer equipped with a TCI cryoprobe and z-axis pulsed-field gradient capability

D91R single mutant of dimeric KaiB (thioredoxin-like fold)

G89A,D91R double mutant of KaiB bound to CI

Sample: [N15,C13,H2]-FLAG-TeKaiB-1-94-Y8A-Y94A (550 uM) + FLAG-TeKaiC-17-247-R41A-K173A-FLAG (560 uM);
Buffer: 20 mM Tris, 1.5 mM MgCl2, 1.5 mM ADP, 75 mM NaCl, pH 7.0, 10 uM DSS, 0.02% NaN3, 95% H2O/5% D2O, 1X Protease Inhibitor Cocktail;
Volume: 380 uL;
Tube: shaped tube

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax