SKELEMIN ASSOCIATION WITH ALFA2B,BETTA3 INTEGRIN: A STRUCTURAL MODEL

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Skelemin association with αIIbβ3 integrin: a structural model.

Over the last two decades, our knowledge concerning intracellular events that
regulate integrin's affinity to their soluble ligands has significantly
improved. However, the mechanism of adhesion-induced integrin clustering and
development of focal complexes, which could further mature to form focal
adhesions, still remains under-investigated. Here we present a structural model
of tandem IgC2 domains of skelemin in complex with the cytoplasmic tails of
integrin alfa2b/betta3. The model of this tertiary assembly is generated based
upon NMR data and illuminates a potential link between the essential cell
adhesion receptors and myosin filaments. This connection may serve as a basis
for generating the mechanical forces necessary for cell migration and
remodeling.

ARIA2: automated NOE assignment and data integration in NMR structure calculation
The CCPN NMR assignment and data analysis application
NMRPipe: a multidimensional spectral processing system based on UNIX pipes
The Xplor-NIH NMR Molecular Structure Determination Package
NOE assignment and structure calculation
Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Department of Biochemistry, Cambridge CB2 1GA, UK