Conformation and dynamics of the periplasmic membrane-protein chaperone complexes OmpX Skp and tOmpA Skp

Conformation and dynamics of the periplasmic membrane-protein-chaperone complexes OmpX-Skp and tOmpA-Skp.

native processed E. coli Skp (rseidues 21 - 162) + initiator methionine + hexa-histidin tag + thrombin cleavage site (coming from pET28b)

native processed transmembrane domain of E. coli OmpA (residues 22 - 197) + initiator methionine

Crystal Structure Of The Periplasmic Chaperone Skp

periplasmic chaperone [Escherichia coli str. K12 substr. W3110]

histone-like protein HlpA [Escherichia coli O157:H7 str. Sakai]

conserved hypothetical protein [Escherichia coli SE11]

periplasmic chaperone [Escherichia coli O26:H11 str. 11368]

periplasmic chaperone [Escherichia coli O103:H2 str. 12009]

chaperone protein skp [Escherichia coli LF82]

periplasmic chaperone, subunit of Skp periplasmic chaperone [Escherichia coli BL21(DE3)]

periplasmic chaperone [Escherichia coli IAI1]

periplasmic chaperone [Escherichia coli S88]

17K protein (gtg start codon) [Escherichia coli]

periplasmic chaperone [Escherichia coli str. K-12 substr. MG1655]

histone-like protein, located in outer membrane or nucleoid [Escherichia coli O157:H7 str. EDL933]

histone-like protein [Shigella flexneri 2a str. 301]

periplasmic chaperone [Escherichia coli O157:H7 str. Sakai]

periplasmic chaperone [Shigella flexneri 2a str. 301]

chaperone protein Skp [Escherichia coli]

RecName: Full=Chaperone protein Skp; AltName: Full=DNA-binding 17 kDa protein; AltName: Full=Histone-like protein HLP-1; AltNam

RecName: Full=Chaperone protein Skp; Flags: Precursor

Skp 15N-1H-backbone chemical shifts when bound to its substrate tOmpA

15N 1H chemical shifts were derived from the previous assigned Skp/OmpX as well as apo-Skp. Therefore no 3D assignment experiments were measured.

Corresponding chemical shifts of Skp when bound to its substrate OmpX

Corresponding chemical shifts of apo-SKP

Protein samples were produced as a 3:1 ratio (Skp monomer per tOmpA)