Magnesium bound form of UVI31+ from C. reinhardtii

insert at center of experimental sample tube

¹H, ¹³C and ¹⁵N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii.

Endonuclease activity and acting as bifunctional nucleases

predicted protein [Chlamydomonas reinhardtii]

Biochemical effect of S-67 phosphorylation on UVI31+ from C. reinhardtii

90% H2O and 10% 2H2O containing 50 mM sodium phosphate (pH 6.4) and 100 mM NaCl. The protein concentrations were 0.6 mM. NMR experiments were recorded on a Bruker Avance 800 MHz NMR spectrometer equipped with a 5 mm triple-resonance cryogenic probe. Experiments recorded at 298 K with uniformly 15N-labeled-S55A- UVI31+ included sensitivity-enhanced 2D [15N 1H] HSQC using water-flipback for minimizing water saturation.