Solution NMR assignments of the phosphorylation-mimicking mutant of V5 domain from Protein Kinase C alpha

T638E/S657E V5 domain of Protein Kinase C alpha

Crystal structure of PKC alpha in complex with NVP-AEB071

The C-terminal V5 domain of Protein Kinase Cα is intrinsically disordered, with propensity to associate with a membrane mimetic.

Residue 673 (HSL) is a non-standard Homoserine-lactone that resulted from CNBr cleavage at Methionine residue

Crystal Structure Of Human Protein Kinase C Alpha In Complex With Compound 28 ((r)-6-((3s,4s)-1,3-dimethyl-piperidin-4-yl)-7-(2

In order to accommodate heterologous expression, the DNA sequence which encodes
the amino acid sequence of V5 domain was codon optimized base on the codon usage
of E. coli. The input amino acid sequence of V5 domain is completely identical
among Homo sapiens, Mus musculus and Rattus norvegicus.

V5 domain from Protein Kinase C alpha, phosphorylation-mimicking mutant

V5 domain of Protein Kinase C alpha, in complex with DPC micelles

T638E/S657E V5 domain of Protein Kinase C alpha, in complex with DPC micelles

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax