Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1

G1 and S2 are non-native residues that remain from thrombin cleavage of the affinity tag.
Residues Y3-N73 correspond to murine LMO4 residues 77-147.
Residues G74-S81 correspond to a synthetic G/S linker peptide.
Residues I82-K96 corresond to murine DEAF1 residues 404-418.

Lmo4-lim2 In Complex With Deaf1 (404-418)

unnamed protein product, partial [Tetraodon nigroviridis]

LIM domain only 4 [Xenopus (Silurana) tropicalis]

LIM domain transcription factor LMO4 [Mus musculus]

nuclear LIM-only 4 protein [Mus musculus]

LIM domain transcription factor LMO4.2 [Xenopus (Silurana) tropicalis]

LIM domain transcription factor LMO4 [Rattus norvegicus]

LIM domain transcription factor LMO4 [Bos taurus]

LIM domain only 4, gene 1 [Xenopus laevis]

LIM domain transcription factor LMO4 [Sus scrofa]

RecName: Full=LIM domain transcription factor LMO4; AltName: Full=Breast tumor autoantigen; AltName: Full=LIM domain only prote

RecName: Full=LIM domain transcription factor LMO4; AltName: Full=LIM domain only protein 4; Short=LMO-4 [Bos taurus]

RecName: Full=LIM domain transcription factor LMO4.2; AltName: Full=LIM domain only protein 4.2; Short=LMO-4.2

TPA: LIM domain transcription factor LMO4 [Bos taurus]

add chloramphenicol 35 micrograms/mL and EDTA-free protease inhibitor tablet (1 tablet/50 mL).
Then freeze-dry prior to resuspension for solution NMR