Backbone 15N, 1H, and CA Chemical Shift Assignments for Y254L/T257A IkappaBalpha (67-287)

Long-range effects and functional consequences of stabilizing mutations in the ankyrin repeat domain of IκBα.

This is the native human IkappaBalpha (67-287), except with consensus mutations at Y254 and T257.

Inhibitor of transcription factor NFkappaB

unnamed protein product [Macaca fascicularis]

nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha [synthetic construct]

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha [Homo sapiens]

NF-kappa-B inhibitor alpha [Macaca mulatta]

uncharacterized protein LOC101867458 [Macaca fascicularis]

NF-kappa-B inhibitor alpha [Homo sapiens]

PREDICTED: NF-kappa-B inhibitor alpha [Callithrix jacchus]

PREDICTED: NF-kappa-B inhibitor alpha [Pongo abelii]

RecName: Full=NF-kappa-B inhibitor alpha; AltName: Full=I-kappa-B-alpha; Short=IkB-alpha; Short=IkappaBalpha; AltName: Full=Maj

The effects of consensus mutations, Y254L/T257A and C186P/A220P, on the structure
and dynamics of the AR domain of IkappaBalpha were investigated by NMR.

Conventional assignment experiments were used to confirm and add to assignments
transferred to WT, YLTA, and CPAP IkappaBalpha(67-287) from the assignments of
WT IkappaBalpha (67-207) and NFkappaB-bound WT IkappaBalpha (67-287). Specific
15N-labelled HSQCs were also used to confirm assignment transfers.

These sample conditions were used for all NMR samples.

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

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