Backbone resonance assignments of the homotetrameric (48 kD) copper sensor CsoR from Geobacillus thermodenitrificans in the apo- and Cu(I)-bound states: insights into copper-mediated allostery.

The wild type residue Threonine position 2 was replaced by an Alanine at position 2 upon vector construction.

Cu(I) loaded form of geobacillus thermodenitrificans CsoR

115 mM NaCl, 5mM TCEP, 3mM EDTA, 20mM ARG, 20 mM GLU