NMR structure of the PhyRSL-NepR complex from Sphingomonas sp. Fr1

Proceedings of the National Academy of Sciences of the United States of America
Structural basis for sigma factor mimicry in the general stress response of Alphaproteobacteria.

From residue 144 to 157, amino acid residues of thrombin cleavage site + his tag

From residue 57-62, amino acids of the thrombin cleavage site

Phyllosphere-induced regulator PhyR [Sphingomonas taxi]

two-component response regulator [Sphingomonas melonis]

Alphaproteobacteria living in the phyllosphere

equipped with a triple-channel probehead and actively shielded gradients.

Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman